dc.contributor.author |
Minlebaev M. |
|
dc.contributor.author |
Valeeva G. |
|
dc.contributor.author |
Tcheremiskine V. |
|
dc.contributor.author |
Coustillier G. |
|
dc.contributor.author |
Khazipov R. |
|
dc.date.accessioned |
2018-09-18T20:24:15Z |
|
dc.date.available |
2018-09-18T20:24:15Z |
|
dc.date.issued |
2013 |
|
dc.identifier.issn |
1662-5102 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/139527 |
|
dc.description.abstract |
We present a novel non-invasive technique to measure the polarity of GABAergic responses based on cell-attached recordings of currents activated by laser-uncaging of GABA. For these recordings, a patch pipette was filled with a solution containing RuBi-GABA, and GABA was released from this complex by a laser beam conducted to the tip of the patch pipette via an optic fiber. In cell-attached recordings from neocortical and hippocampal neurons in postnatal days P2-5 rat brain slices in vitro, we found that laser-uncaging of GABA activates integral cell-attached currents mediated by tens of GABA(A) channels. The initial response was inwardly directed, indicating a depolarizing response to GABA. The direction of the initial response was dependent on the pipette potential and analysis of its slope-voltage relationships revealed a depolarizing driving force of +11 mV for the currents through G. Current-voltage relationships of the currents evoked by Rubi-GABA uncaging using voltage-ramps at the peak of responses not only revealed a bumetanide-sensitive depolarizing reversal potential of the GABA(A) receptor mediated responses, but also showed a strong voltage-dependent hysteresis. Upon desensitization of the uncaged-GABA response, current-voltage relationships of the currents through single GABA(A) channels revealed depolarizing responses with the driving force values similar to those obtained for the initial response. Thus, cell-attached recordings of the responses evoked by local intrapipette GABA uncaging are suitable to assess the polarity of the GABA(A)-Rs mediated signals in small cell compartments. © 2013 Minlebaev, Valeeva, Tcheremiskine, Coustillier and Khazipov. |
|
dc.relation.ispartofseries |
Frontiers in Cellular Neuroscience |
|
dc.subject |
Cell-attach |
|
dc.subject |
GABA |
|
dc.subject |
Inhibitory postsynaptic potentials |
|
dc.subject |
Patch-clamp |
|
dc.subject |
Rubi-GABA |
|
dc.subject |
Uncaged GABA |
|
dc.title |
Cell-attached recordings of responses evoked by photorelease of GABA in the immature cortical neurons |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
MAY |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.source.id |
SCOPUS16625102-2013--SID84878105012 |
|