Показать сокращенную информацию
dc.contributor.author | Fakhrullin R. | |
dc.contributor.author | Vinter V. | |
dc.contributor.author | Zamaleeva A. | |
dc.contributor.author | Matveeva M. | |
dc.contributor.author | Kourbanov R. | |
dc.contributor.author | Temesgen B. | |
dc.contributor.author | Ishmuchametova D. | |
dc.contributor.author | Abramova Z. | |
dc.contributor.author | Konovalova O. | |
dc.contributor.author | Salakhov M. | |
dc.date.accessioned | 2018-09-18T20:24:08Z | |
dc.date.available | 2018-09-18T20:24:08Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 1618-2642 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/139504 | |
dc.description.abstract | We report the development of a novel quartz crystal microbalance immunosensor with the simultaneous measurement of resonance frequency and motional resistance for the detection of antibodies to double-stranded DNA (dsDNA). The immobilization of poly(l-lysine) and subsequent complexation with DNA resulted in formation of a sensitive dsDNA-containing nanofilm on the surface of a gold electrode. Atomic force microscopy has been applied for the characterization of a poly(l-lysine)-DNA film. After the blocking with bovine serum albumin, the immunosensor in flow-injection mode was used to detect the antibodies to dsDNA in purified protein solutions of antibodies to dsDNA and to single-stranded DNA, monoclonal human immunoglobulin G, DNase I and in blood serum of patients with bronchial asthma and systemic lupus erythematosus. Experimental results indicate high sensitivity and selectivity of the immunosensor. [Figure not available: see fulltext.]. © Springer-Verlag 2007. | |
dc.relation.ispartofseries | Analytical and Bioanalytical Chemistry | |
dc.subject | Antibodies to double-stranded DNA | |
dc.subject | Atomic force microscopy | |
dc.subject | Bronchial asthma | |
dc.subject | Immunosensor | |
dc.subject | Quartz crystal microbalance | |
dc.subject | Systemic lupus erythematosus | |
dc.title | Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA | |
dc.type | Conference Paper | |
dc.relation.ispartofseries-issue | 2 | |
dc.relation.ispartofseries-volume | 388 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 367 | |
dc.source.id | SCOPUS16182642-2007-388-2-SID34247562221 |