dc.contributor.author |
Fakhrullin R. |
|
dc.contributor.author |
Vinter V. |
|
dc.contributor.author |
Zamaleeva A. |
|
dc.contributor.author |
Matveeva M. |
|
dc.contributor.author |
Kourbanov R. |
|
dc.contributor.author |
Temesgen B. |
|
dc.contributor.author |
Ishmuchametova D. |
|
dc.contributor.author |
Abramova Z. |
|
dc.contributor.author |
Konovalova O. |
|
dc.contributor.author |
Salakhov M. |
|
dc.date.accessioned |
2018-09-18T20:24:08Z |
|
dc.date.available |
2018-09-18T20:24:08Z |
|
dc.date.issued |
2007 |
|
dc.identifier.issn |
1618-2642 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/139504 |
|
dc.description.abstract |
We report the development of a novel quartz crystal microbalance immunosensor with the simultaneous measurement of resonance frequency and motional resistance for the detection of antibodies to double-stranded DNA (dsDNA). The immobilization of poly(l-lysine) and subsequent complexation with DNA resulted in formation of a sensitive dsDNA-containing nanofilm on the surface of a gold electrode. Atomic force microscopy has been applied for the characterization of a poly(l-lysine)-DNA film. After the blocking with bovine serum albumin, the immunosensor in flow-injection mode was used to detect the antibodies to dsDNA in purified protein solutions of antibodies to dsDNA and to single-stranded DNA, monoclonal human immunoglobulin G, DNase I and in blood serum of patients with bronchial asthma and systemic lupus erythematosus. Experimental results indicate high sensitivity and selectivity of the immunosensor. [Figure not available: see fulltext.]. © Springer-Verlag 2007. |
|
dc.relation.ispartofseries |
Analytical and Bioanalytical Chemistry |
|
dc.subject |
Antibodies to double-stranded DNA |
|
dc.subject |
Atomic force microscopy |
|
dc.subject |
Bronchial asthma |
|
dc.subject |
Immunosensor |
|
dc.subject |
Quartz crystal microbalance |
|
dc.subject |
Systemic lupus erythematosus |
|
dc.title |
Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA |
|
dc.type |
Conference Paper |
|
dc.relation.ispartofseries-issue |
2 |
|
dc.relation.ispartofseries-volume |
388 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
367 |
|
dc.source.id |
SCOPUS16182642-2007-388-2-SID34247562221 |
|