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Sensitivity of acute myeloid leukemia Kasumi-1 cells to binase toxic action depends on the expression of KIT and AML1-ETO oncogenes

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dc.contributor.author Mitkevich V.
dc.contributor.author Petrushanko I.
dc.contributor.author Spirin P.
dc.contributor.author Fedorova T.
dc.contributor.author Kretova O.
dc.contributor.author Tchurikov N.
dc.contributor.author Prassolov V.
dc.contributor.author Ilinskaya O.
dc.contributor.author Makarov A.
dc.date.accessioned 2018-09-18T20:23:09Z
dc.date.available 2018-09-18T20:23:09Z
dc.date.issued 2011
dc.identifier.issn 1538-4101
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/139338
dc.description.abstract Some RNases selectively attack malignant cells, triggering an apoptotic response and therefore are considered as alternative chemotherapeutic drugs. Here, we studied the effects of Bacillus intermedius RNase (binase) on murine myeloid progenitor cells FDC-P1, transduced FDC-P1 cells ectopically expressing mutated human KIT N822K oncogene and/or human AML1-ETO oncogene and human leukemia Kasumi-1 cells expressing both of these oncogenes. Expression of both KIT and AML1-ETO oncogenes makes FDC-P1 cells sensitive to the toxic effects of binase. Kasumi-1 cells were the most responsive to the toxic actions of binase among the cell lines used in this work with an IC 50 value of 0.56 μM. Either blocking the functional activity of the KIT protein with imatinib or knocking-down oncogene expression using lentiviral vectors producing shRNA against AML1-ETO or KIT eliminated the sensitivity of Kasumi-1 cells to binase toxic action and promoted their survival, even in the absence of KIT-dependent proliferation and antiapoptotic pathways. Here, we provide evidence that the cooperative effect of the expression of mutated KIT and AML1-ETO oncogenes is crucial for selective toxic action of binase on malignant cells. These findings can facilitate clinical applications of binase, providing a useful screen based on the presence of the corresponding target oncogenes in malignant cells. © 2011 Landes Bioscience.
dc.relation.ispartofseries Cell Cycle
dc.subject Apoptosis
dc.subject Cytotoxic RNase
dc.subject Imatinib
dc.subject Myeloid progenitor cells
dc.subject Oncogenes
dc.subject ShRNA
dc.title Sensitivity of acute myeloid leukemia Kasumi-1 cells to binase toxic action depends on the expression of KIT and AML1-ETO oncogenes
dc.type Article
dc.relation.ispartofseries-issue 23
dc.relation.ispartofseries-volume 10
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 4090
dc.source.id SCOPUS15384101-2011-10-23-SID82855169516


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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