The novel adams-like microbial metalloendopeptidase

Abstract

Heterologous gene expression of the extracellular minor metalloendopeptidase of Bacillus pumilus 3-19 (AN EU678894) in the protease-deficient B. pumilus 3-19 strain has been studied. The enzyme fraction in the total pool of B. pumilus 3-19 proteases is less than 8%. The enzyme was isolated from the culture liquid of the recombinant strain and purified to a homogeneous state; its primary structure was determined and its physicochemical properties were investigated. The secreted metalloendopeptidase of B. pumilus 3-19 is the first prokaryotic homologue of the eukaryotic adamalysin/reprolysin protein family.© Pleiades Publishing, Ltd., 2012.