dc.contributor.author |
Usachev K. |
|
dc.contributor.author |
Efimov S. |
|
dc.contributor.author |
Kolosova O. |
|
dc.contributor.author |
Klochkova E. |
|
dc.contributor.author |
Aganov A. |
|
dc.contributor.author |
Klochkov V. |
|
dc.date.accessioned |
2018-09-18T20:10:21Z |
|
dc.date.available |
2018-09-18T20:10:21Z |
|
dc.date.issued |
2015 |
|
dc.identifier.issn |
0925-2738 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/137237 |
|
dc.description.abstract |
© 2015 Springer Science+Business Media Dordrecht. A tendency to dimerize in the presence of lipids was found for the protegrin. The dimer formation by the protegrin-1 (PG-1) is the first step for further oligomeric membrane pore formation. Generally there are two distinct model of PG-1 dimerization in either a parallel or antiparallel β-sheet. But despite the wealth of data available today, protegrin dimer structure and pore formation is still not completely understood. In order to investigate a more detailed dimerization process of PG-1 and if it will be the same for another type of protegrins, in this work we used a high-resolution NMR spectroscopy for structure determination of protegrin-3 (RGGGL-CYCRR-RFCVC-VGR) in the presence of perdeuterated DPC micelles and demonstrate that PG-3 forms an antiparallel NCCN dimer with a possible association of these dimers. This structural study complements previously published solution, solid state and computational studies of PG-1 in various environments and validate the potential of mean force simulations of PG-1 dimers and association of dimers to form octameric or decameric β-barrels. |
|
dc.relation.ispartofseries |
Journal of Biomolecular NMR |
|
dc.subject |
Antimicrobial peptide |
|
dc.subject |
Dimer |
|
dc.subject |
DPC micelle |
|
dc.subject |
NMR |
|
dc.subject |
Protegrin |
|
dc.subject |
Structure |
|
dc.title |
Antimicrobial peptide protegrin-3 adopt an antiparallel dimer in the presence of DPC micelles: A high-resolution NMR study |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
1 |
|
dc.relation.ispartofseries-volume |
62 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
71 |
|
dc.source.id |
SCOPUS09252738-2015-62-1-SID84939989258 |
|