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dc.contributor.author | Cabrera-Fuentes H. | |
dc.contributor.author | Lopez M. | |
dc.contributor.author | McCurdy S. | |
dc.contributor.author | Fischer S. | |
dc.contributor.author | Meiler S. | |
dc.contributor.author | Baumer Y. | |
dc.contributor.author | Galuska S. | |
dc.contributor.author | Preissner K. | |
dc.contributor.author | Boisvert W. | |
dc.date.accessioned | 2018-09-18T20:08:49Z | |
dc.date.available | 2018-09-18T20:08:49Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0340-6245 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/136966 | |
dc.description.abstract | © Schattauer 2015. Monocytes/macrophages respond to external stimuli with rapid changes in the expression of numerous inflammation-related genes to undergo polarisation towards the M1 (pro-inflammatory) or M2 (antiinflammatory) phenotype. We have previously shown that, independently of Toll-like receptor activation, extracellular RNA (eRNA) could exert pro-thrombotic and pro-inflammatory properties in the cardiovascular system to provoke cytokine mobilisation. Here, mouse bone marrow-derived-macrophages (BMDM) differentiated with mouse macrophage-colony-stimulating factor (M-CSF) were found to be skewed towards the M1 phenotype when exposed to eRNA. This resulted in up-regulated expression of inflammatory markers such as Tnf-α and Il-6, together with Il-12 and iNOS, whereas anti-inflammatory genes such as chitinase-like proteins (Ym1/2) and macrophage mannose receptor-2 (Cd206) were significantly down-regulated. Human peripheral blood monocytes were treated with eRNA and analysed by micro-array analysis of the whole human genome, revealing an up-regulation of 79 genes by at least four-fold; 27 of which are related to signal transduction and 15 genes associated with inflammatory response. In accordance with the proposed actions of eRNA as a pro-inflammatory “alarm signal”, these data shed light on the role of eRNA in the context of chronic inflammatory diseases such as atherosclerosis. | |
dc.relation.ispartofseries | Thrombosis and Haemostasis | |
dc.subject | Extracellular RNA | |
dc.subject | Gene expression | |
dc.subject | Inflammation | |
dc.subject | Microarray technology | |
dc.subject | Monocyte/macrophage polarization | |
dc.title | Regulation of monocyte/macrophage polarisation by extracellular RNA | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 3 | |
dc.relation.ispartofseries-volume | 113 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 473 | |
dc.source.id | SCOPUS03406245-2015-113-3-SID84924193206 |