Kazan Federal University Digital Repository

A hydrophobic segment of some cytotoxic ribonucleases

Show simple item record

dc.contributor.author Shirshikov F.
dc.contributor.author Cherepnev G.
dc.contributor.author Ilinskaya O.
dc.contributor.author Kalacheva N.
dc.date.accessioned 2018-09-18T20:08:42Z
dc.date.available 2018-09-18T20:08:42Z
dc.date.issued 2013
dc.identifier.issn 0306-9877
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/136949
dc.description.abstract The exact mechanism by which cytotoxic ribonucleases reach the cytosol of tumor cells remains unclear. The interaction of ribonucleases with a lipid bilayer is involved in the translocation of ribonucleases across the endosomal membrane. Here, we aimed to study the hydropathy character of toxic antitumor ribonucleases (bovine seminal ribonuclease and binase) and two non-toxic ribonucleases (bovine pancreatic ribonuclease and human pancreatic ribonuclease) by sliding-window hydrophobicity analysis. Comparative hydropathy plot analysis of the non-toxic pancreatic ribonucleases and their toxic variants was also performed. The data obtained indicate that some cytotoxic ribonucleases have a hydrophobic segment, which is sterically available for the hydrophobic interaction with a tumor cell membrane and endosomal membrane. After dissociation, subunits of dimeric ribonucleases are probably capable of thermodynamically favorable interaction with the interfacial region of a lipid bilayer. Remarkably the hydrophobic segment is not identified in the amino acid sequences of non-toxic ribonucleases. The paper describes the hydrophobic properties of toxic RNases that are essential for both the model of a lipid-protein interaction and the cytotoxicity mechanism unraveling. © 2013 Elsevier Ltd.
dc.relation.ispartofseries Medical Hypotheses
dc.title A hydrophobic segment of some cytotoxic ribonucleases
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 81
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 328
dc.source.id SCOPUS03069877-2013-81-2-SID84880047111


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics