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Ribonuclease binase apoptotic signature in leukemic Kasumi-1 cells

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dc.contributor.author Mitkevich V.
dc.contributor.author Kretova O.
dc.contributor.author Petrushanko I.
dc.contributor.author Burnysheva K.
dc.contributor.author Sosin D.
dc.contributor.author Simonenko O.
dc.contributor.author Ilinskaya O.
dc.contributor.author Tchurikov N.
dc.contributor.author Makarov A.
dc.date.accessioned 2018-09-18T20:08:16Z
dc.date.available 2018-09-18T20:08:16Z
dc.date.issued 2013
dc.identifier.issn 0300-9084
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/136873
dc.description.abstract Cytotoxic exogenous RNases triggering apoptotic response in malignant cells have potential as anticancer drugs; surprisingly, detailed characterization of the RNase-induced apoptosis has not been conducted so far. Here we show that a cytotoxic RNase from Bacillus intermedius (binase) induces extrinsic and intrinsic apoptotic pathways in leukemic Kasumi-1 cells. The experiments were performed using TaqMan Array Human Apoptosis 96-well Plate for gene expression analysis, and flow cytometry. Cytometric studies demonstrated dissipation of the mitochondrial membrane potential, opening of mitochondrial permeability transition pores, activation of caspases, increase of intracellular Ca 2+ and decrease of reactive oxygen species levels. We found that expression of 62 apoptotic genes is up-regulated, including 16 genes that are highly up-regulated, and only one gene was found to be down-regulated. The highest, 16 fold increase of the expression level was observed for TNF gene. Highly up-regulated genes also include the non-canonical NF-κB signaling pathway and inflammatory caspases 1,4. The obtained results suggest that binase induces evolutionary acquired cellular response to a microbial agent and triggers unusual apoptosis pathway. © 2013 Elsevier Masson SAS. All rights reserved.
dc.relation.ispartofseries Biochimie
dc.subject Apoptosis
dc.subject Binase
dc.subject Cytotoxic RNase
dc.subject Gene expression
dc.subject Kasumi-1 cells
dc.title Ribonuclease binase apoptotic signature in leukemic Kasumi-1 cells
dc.type Article
dc.relation.ispartofseries-issue 6
dc.relation.ispartofseries-volume 95
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1344
dc.source.id SCOPUS03009084-2013-95-6-SID84877765977


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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