dc.contributor.author |
Rizvanov A. |
|
dc.contributor.author |
Kiyasov A. |
|
dc.contributor.author |
Gaziziov I. |
|
dc.contributor.author |
Yilmaz T. |
|
dc.contributor.author |
Kaligin M. |
|
dc.contributor.author |
Andreeva D. |
|
dc.contributor.author |
Shafigullina A. |
|
dc.contributor.author |
Guseva D. |
|
dc.contributor.author |
Kiselev S. |
|
dc.contributor.author |
Matin K. |
|
dc.contributor.author |
Palotás A. |
|
dc.contributor.author |
Islamov R. |
|
dc.date.accessioned |
2018-09-18T20:07:03Z |
|
dc.date.available |
2018-09-18T20:07:03Z |
|
dc.date.issued |
2008 |
|
dc.identifier.issn |
0197-0186 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/136661 |
|
dc.description.abstract |
Genetically modified mono-nuclear cell fraction from human umbilical cord blood (HUCB) expressing human vascular endothelial growth factor (VEGF) and mouse neural L1 cell adhesion molecule (L1CAM) were used for gene-stem cell therapy of transgenic G93A mice adopted as an animal amyotrophic lateral sclerosis (ALS) model. We generated non-viral plasmid constructs, expressing human VEGF165 (pcDNA-VEGF) and mouse neural L1 cell adhesion molecule (pcDNA-mL1CAM). Mono-nuclear fraction of HUCB cells were transiently transfected by electro-poration with a mixture of expression plasmids (pcDNA-VEGF + pcDNA-mL1CAM). Sixteen transgenic female and male mice were randomly assigned to three groups: (1) transplantation of genetically modified HUCB cells expressing L1 and VEGF (n = 6), (2) transplantation of un-transfected HUCB cells (n = 5), and (3) control group (n = 5). In first two experimental groups 1 × 106 cells were injected retro-orbitally in pre-symptomatic 22-25-week-old G93A mice. Our results demonstrate that HUCB cells successfully grafted into nervous tissue of ALS mice and survived for over 3 months. Therefore, genetically modified HUCB cells migrate in the spinal cord parenchyma, proliferate, but instead of transforming into nerve cells, they differentiate into endothelial cells forming new blood vessels. We propose that: (A) expression of mouse neural L1CAM is responsible for increased homing and subsequent proliferation of transplanted cells at the site of neuro-degeneration, (B) expression of human VEGF directs HUCB cell differentiation into endothelial cells, and (C) neuro-protective effect may stem from the delivery of various neuro-trophic factors from newly formed blood vessels. © 2008 Elsevier Ltd. All rights reserved. |
|
dc.relation.ispartofseries |
Neurochemistry International |
|
dc.subject |
Amyotrophic lateral sclerosis |
|
dc.subject |
Human umbilical cord blood |
|
dc.subject |
L1CAM |
|
dc.subject |
Stem cell |
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dc.subject |
VEGF |
|
dc.title |
Human umbilical cord blood cells transfected with VEGF and L1CAM do not differentiate into neurons but transform into vascular endothelial cells and secrete neuro-trophic factors to support neuro-genesis-a novel approach in stem cell therapy |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
6-8 |
|
dc.relation.ispartofseries-volume |
53 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
389 |
|
dc.source.id |
SCOPUS01970186-2008-53-68-SID56649118944 |
|