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Human umbilical cord blood cells transfected with VEGF and L1CAM do not differentiate into neurons but transform into vascular endothelial cells and secrete neuro-trophic factors to support neuro-genesis-a novel approach in stem cell therapy

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dc.contributor.author Rizvanov A.
dc.contributor.author Kiyasov A.
dc.contributor.author Gaziziov I.
dc.contributor.author Yilmaz T.
dc.contributor.author Kaligin M.
dc.contributor.author Andreeva D.
dc.contributor.author Shafigullina A.
dc.contributor.author Guseva D.
dc.contributor.author Kiselev S.
dc.contributor.author Matin K.
dc.contributor.author Palotás A.
dc.contributor.author Islamov R.
dc.date.accessioned 2018-09-18T20:07:03Z
dc.date.available 2018-09-18T20:07:03Z
dc.date.issued 2008
dc.identifier.issn 0197-0186
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/136661
dc.description.abstract Genetically modified mono-nuclear cell fraction from human umbilical cord blood (HUCB) expressing human vascular endothelial growth factor (VEGF) and mouse neural L1 cell adhesion molecule (L1CAM) were used for gene-stem cell therapy of transgenic G93A mice adopted as an animal amyotrophic lateral sclerosis (ALS) model. We generated non-viral plasmid constructs, expressing human VEGF165 (pcDNA-VEGF) and mouse neural L1 cell adhesion molecule (pcDNA-mL1CAM). Mono-nuclear fraction of HUCB cells were transiently transfected by electro-poration with a mixture of expression plasmids (pcDNA-VEGF + pcDNA-mL1CAM). Sixteen transgenic female and male mice were randomly assigned to three groups: (1) transplantation of genetically modified HUCB cells expressing L1 and VEGF (n = 6), (2) transplantation of un-transfected HUCB cells (n = 5), and (3) control group (n = 5). In first two experimental groups 1 × 106 cells were injected retro-orbitally in pre-symptomatic 22-25-week-old G93A mice. Our results demonstrate that HUCB cells successfully grafted into nervous tissue of ALS mice and survived for over 3 months. Therefore, genetically modified HUCB cells migrate in the spinal cord parenchyma, proliferate, but instead of transforming into nerve cells, they differentiate into endothelial cells forming new blood vessels. We propose that: (A) expression of mouse neural L1CAM is responsible for increased homing and subsequent proliferation of transplanted cells at the site of neuro-degeneration, (B) expression of human VEGF directs HUCB cell differentiation into endothelial cells, and (C) neuro-protective effect may stem from the delivery of various neuro-trophic factors from newly formed blood vessels. © 2008 Elsevier Ltd. All rights reserved.
dc.relation.ispartofseries Neurochemistry International
dc.subject Amyotrophic lateral sclerosis
dc.subject Human umbilical cord blood
dc.subject L1CAM
dc.subject Stem cell
dc.subject VEGF
dc.title Human umbilical cord blood cells transfected with VEGF and L1CAM do not differentiate into neurons but transform into vascular endothelial cells and secrete neuro-trophic factors to support neuro-genesis-a novel approach in stem cell therapy
dc.type Article
dc.relation.ispartofseries-issue 6-8
dc.relation.ispartofseries-volume 53
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 389
dc.source.id SCOPUS01970186-2008-53-68-SID56649118944


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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