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Binding and purification of plasmid DNA using multi-layered carbon nanotubes

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dc.contributor.author Shakhmaeva I.
dc.contributor.author Bulatov E.
dc.contributor.author Bondar O.
dc.contributor.author Saifullina D.
dc.contributor.author Culha M.
dc.contributor.author Rizvanov A.
dc.contributor.author Abdullin T.
dc.date.accessioned 2018-09-18T20:06:50Z
dc.date.available 2018-09-18T20:06:50Z
dc.date.issued 2011
dc.identifier.issn 0168-1656
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/136629
dc.description.abstract We propose a new method for the separation of nucleic acids using multi-layered carbon nanotubes (CNTs) as an adsorbent. According to agarose gel electrophoresis, oxidized water-stable CNTs adsorb certain forms of nucleic acids, such as high molecular weight RNA, chromosomal DNA, linear and denatured forms of plasmid DNA. However, CNTs do not adsorb supercoiled form of plasmid DNA. Nucleic acids bound to CNTs can be readily removed by centrifugation whereas supercoiled plasmid DNA remains in solution. Upon the addition of divalent metal ions supercoiled plasmid DNA forms relatively stable complexes with CNTs due to chelation. Thus, new details about association of nucleic acids with CNTs were revealed and stoichiometry of the complexes was estimated. Our results can be used for fine purification of supercoiled plasmid DNA for gene therapy applications as well as manipulation of nucleic acids for biosensor design. © 2011 Elsevier B.V.
dc.relation.ispartofseries Journal of Biotechnology
dc.subject Adsorbents
dc.subject Biosensors
dc.subject Carbon nanotubes
dc.subject Gene therapy
dc.subject Nucleic acid separation
dc.subject Plasmid DNA
dc.title Binding and purification of plasmid DNA using multi-layered carbon nanotubes
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 152
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 102
dc.source.id SCOPUS01681656-2011-152-3-SID79952898401


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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