dc.contributor.author |
Fedorova K. |
|
dc.contributor.author |
Scharafutdinov I. |
|
dc.contributor.author |
Turbina E. |
|
dc.contributor.author |
Bogachev M. |
|
dc.contributor.author |
Ilinskaja O. |
|
dc.contributor.author |
Kayumov A. |
|
dc.date.accessioned |
2018-09-18T20:06:14Z |
|
dc.date.available |
2018-09-18T20:06:14Z |
|
dc.date.issued |
2013 |
|
dc.identifier.issn |
0026-8933 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/136532 |
|
dc.description.abstract |
The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C-terminus controls the DNA-binding activity in a full-length TnrA. © 2013 Pleiades Publishing, Ltd. |
|
dc.relation.ispartofseries |
Molecular Biology |
|
dc.subject |
Bacillus subtilis |
|
dc.subject |
dimerization |
|
dc.subject |
transcription factor TnrA |
|
dc.title |
C-terminus of transcription factor TnrA from Bacillus subtilis controls DNA-binding domain activity but is not required for dimerization |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
2 |
|
dc.relation.ispartofseries-volume |
47 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
293 |
|
dc.source.id |
SCOPUS00268933-2013-47-2-SID84876429954 |
|