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Lipid and fatty acid profiles of Pseudomonas aurantiaca DNA-bound lipids determined by mass spectrometry
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| dc.contributor.author | Zhdanov R. | |
| dc.contributor.author | Kern D. | |
| dc.contributor.author | Lorenz W. | |
| dc.contributor.author | Ibragimova M. | |
| dc.date.accessioned | 2018-09-18T20:06:11Z | |
| dc.date.available | 2018-09-18T20:06:11Z | |
| dc.date.issued | 2015 | |
| dc.identifier.issn | 0026-2617 | |
| dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/136523 | |
| dc.description.abstract | © 2015, Pleiades Publishing, Ltd. An approach used on investigation of the lipid composition of loosely (fraction 1) and tightly (fraction 2) DNA-bound lipids of Pseudomonas aurantiaca cells by electrospray ionization using mass spectrometry (ESI-LC-MS) was used for determination of the lipidome of a prokaryotic cell. Free fatty acids C16:0, C18:1 (fraction 1), C14:0, C16:0, and C18:2 (fraction 2) were detected. Both fractions of DNA-bound lipids were characterized by the presence of phosphatidylglycerol, phosphatidylserine, and lyso-phosphatidylinositol. The alcohol-soluble fraction 1 could also contain phosphatidylcholine and phosphatidylinositol, while fraction 2 probably contained triacylglycerides. Compared to gas chromatography, ESI-LC-MS provides new possibilities for investigation of the nucleoid lipidome, allowing for more detailed investigation of DNA-bound lipids in bacterial cells. | |
| dc.relation.ispartofseries | Microbiology (Russian Federation) | |
| dc.subject | DNA-bound lipids | |
| dc.subject | electrospray ionization | |
| dc.subject | fatty acids | |
| dc.subject | mass spectrometry | |
| dc.subject | Pseudomonas aurantiaca | |
| dc.title | Lipid and fatty acid profiles of Pseudomonas aurantiaca DNA-bound lipids determined by mass spectrometry | |
| dc.type | Article | |
| dc.relation.ispartofseries-issue | 1 | |
| dc.relation.ispartofseries-volume | 84 | |
| dc.collection | Публикации сотрудников КФУ | |
| dc.relation.startpage | 34 | |
| dc.source.id | SCOPUS00262617-2015-84-1-SID84924962101 |
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Публикации сотрудников КФУ Scopus [24551]
Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.