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Temperature dependence of water self-diffusion through lipid bilayers assessed by NMR

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dc.contributor.author Khakimov A.
dc.contributor.author Rudakova M.
dc.contributor.author Doroginitskii M.
dc.contributor.author Filippov A.
dc.date.accessioned 2018-09-18T20:02:11Z
dc.date.available 2018-09-18T20:02:11Z
dc.date.issued 2008
dc.identifier.issn 0006-3509
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135941
dc.description.abstract The temperature dependence of the coefficient of water self-diffusion across plane-parallel multib-ilayers of dioleoylphosphatidylcholine oriented on a glass support was studied in the 20-60°C range by pulsed field gradient NMR. The coefficient for transbilayer diffusion of water proved almost four orders of magnitude smaller than for bulk water, and 10 times smaller than that for lateral diffusion of lipid under the same conditions. The temperature dependence obeyed the Arrhenius law with apparent activation energy of 41 kJ/mol, much higher than that for bulk water (18 kJ/mol). The experimental data were analyzed using the "dissolution-diffusion" model, by simulating water passage through membrane channels, and by examining water exchange in states with different modes of translational mobility, including pore channels and bilayer defects. Each approach could take into account the role of bilayer permeability and assess the apparent activation energy for water diffusion in the hydrophobic part of the bilayer, which proved close to the value for bulk water. Estimates were obtained for water diffusion coefficients in the system, coefficients of bilayer permeability for water, and the influence of bilayer defects on the lateral and transverse diffusion coefficients. © 2008 Pleiades Publishing, Ltd.
dc.relation.ispartofseries Biophysics
dc.subject Lipid bilayer
dc.subject Membrane channels
dc.subject Pulsed field gradient NMR
dc.subject Water diffusion
dc.title Temperature dependence of water self-diffusion through lipid bilayers assessed by NMR
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 53
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 147
dc.source.id SCOPUS00063509-2008-53-2-SID50249155428


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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