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Purification and characterization of a subtilisin-like proteinases secreted in the stationary growth phase of Bacillus amyloliquefaciens H2

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dc.contributor.author Balaban N.
dc.contributor.author Malikova L.
dc.contributor.author Mardanova A.
dc.contributor.author Rudenskaya G.
dc.contributor.author Sharipova M.
dc.date.accessioned 2018-09-18T20:02:05Z
dc.date.available 2018-09-18T20:02:05Z
dc.date.issued 2007
dc.identifier.issn 0006-2979
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135925
dc.description.abstract Proteinases secreted during the early and late stationary phases have been isolated from the culture liquid of Bacillus amyloliquefaciens H2 using CM-cellulose ion-exchange chromatography with subsequent FPLC on a Mono S column. Considering the character of hydrolysis of specific chromogenic substrates and the type of inhibition, these enzymes were identified as subtilisin-like proteinases. The molecular weight of both proteinases is 29 kD. The proteolytic activity of the proteinases secreted during the early and late stationary phases towards the synthetic substrate Z-Ala-Ala-Leu-pNA was maximal at pH 8.5 and 9.0, respectively. The maximal activity of both proteinases was observed at 37°C, and the proteins were stable within the pH range of 7.2-9.5. The subtilisin-like proteinases from B. amyloliquefaciens were shown to catalyze synthesis of peptide bonds. © Nauka/Interperiodica 2007.
dc.relation.ispartofseries Biochemistry (Moscow)
dc.subject Bacillus amyloliquefaciens
dc.subject Properties
dc.subject Purification
dc.subject Subtilisin-like proteinases
dc.title Purification and characterization of a subtilisin-like proteinases secreted in the stationary growth phase of Bacillus amyloliquefaciens H2
dc.type Article
dc.relation.ispartofseries-issue 4
dc.relation.ispartofseries-volume 72
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 459
dc.source.id SCOPUS00062979-2007-72-4-SID34247392443


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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