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Amperometric immunoassay of azinphos-methyl in water and honeybees based on indirect competitive ELISA

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dc.contributor.author Ivanov A.
dc.contributor.author Evtugyn G.
dc.contributor.author Budnikov H.
dc.contributor.author Girotti S.
dc.contributor.author Ghini S.
dc.contributor.author Ferri E.
dc.contributor.author Montoya A.
dc.contributor.author Mercader J.
dc.date.accessioned 2018-09-18T20:01:17Z
dc.date.available 2018-09-18T20:01:17Z
dc.date.issued 2008
dc.identifier.issn 0003-2719
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135806
dc.description.abstract An electrochemical immunosensor based on indirect competitive ELISA technique has been developed and tested for the detection of azinphos-methyl in aqueous solutions and spiked honeybee extracts. The detection of the pesticide was based on competition for binding to monoclonal antibodies with an ovalbumin (OVA) conjugate, followed by the incubation with anti-mouse IgG labeled with horseradish peroxidase, whose activity was measured amperometrically with hydroquinone as the substrate. The sensitivity of the azinphos-methyl assay, estimated as the IC50 value, was found to be 1.2 nmol L-1 (60 min incubation), with a linear range of 0.6-500 nmol L-1 in optimal conditions. The matrix effect on the detection of azinphos-methyl in honeybee extract was found negligible, with the recovery values in the range 92-105%. Copyright © Taylor & Francis Group, LLC.
dc.relation.ispartofseries Analytical Letters
dc.subject Azinphos-methyl
dc.subject Electrochemical immunoassay
dc.subject ELISA
dc.subject Immunosensor
dc.subject Monoclonal antibody
dc.title Amperometric immunoassay of azinphos-methyl in water and honeybees based on indirect competitive ELISA
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 41
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 392
dc.source.id SCOPUS00032719-2008-41-3-SID39049165592


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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