Kazan Federal University Digital Repository

Electrochemical determination of the total antioxidant capacity of human plasma

Show simple item record

dc.contributor.author Ziyatdinova G.
dc.contributor.author Budnikov H.
dc.contributor.author Pogorel'Tzev V.
dc.date.accessioned 2018-09-17T21:36:08Z
dc.date.available 2018-09-17T21:36:08Z
dc.date.issued 2005
dc.identifier.issn 1618-2642
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135198
dc.description.abstract Electrochemical reduction of oxygen at a glassy carbon electrode in a 0.05 mol L-1 solution of (C2H5)4NI in dimethylformamide leads to generation of the superoxide anion-radical. This product of reversible one-electron oxygen reduction reacts with antioxidants, a process which is based on protonation of the anion-radical by the antioxidant. Rate constants of this interaction have been calculated. Human plasma antioxidants also react with electrochemically generated superoxide anion-radical. A voltammetric method is proposed for estimation of the total antioxidant capacity (TAC) of plasma on the basis of on this reaction. The TAC of plasma was also determined using constant-current coulometry with electrogenerated bromine as the active species. A correlation was observed between TAC data obtained by voltammetry ( O•- 2 , in α-tocopherol units) and coulometry (Br2 as titrant). TAC of plasma from patients with purulent infections was determined. Statistically significant differences were found between TAC of patients and control group. Treatment of purulent infections increases the TAC of plasma. So, use of electrochemical methods (voltammetry and coulometry) for determination of TAC can be used for estimation of the effectiveness of treatment. © Springer-Verlag 2005.
dc.relation.ispartofseries Analytical and Bioanalytical Chemistry
dc.subject Plasma
dc.subject Superoxide anion-radical
dc.subject Total antioxidant capacity
dc.subject Voltammetry
dc.title Electrochemical determination of the total antioxidant capacity of human plasma
dc.type Conference Paper
dc.relation.ispartofseries-issue 8
dc.relation.ispartofseries-volume 381
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1546
dc.source.id SCOPUS16182642-2005-381-8-SID18144393804


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics