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Amperometric DNA-peroxidase sensor for the detection of pharmaceutical preparations

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dc.contributor.author Evtugyn G.
dc.contributor.author Goldfarb O.
dc.contributor.author Budnikov H.
dc.contributor.author Ivanov A.
dc.contributor.author Vinter V.
dc.date.accessioned 2018-09-17T21:32:45Z
dc.date.available 2018-09-17T21:32:45Z
dc.date.issued 2005
dc.identifier.issn 1424-3210
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135131
dc.description.abstract Novel DNA-sensor with enzymatic amplification of the signal has been developed on the base of glassy carbon electrode modified with ds-DNA and horseradish peroxidase (HRP). Phenothiazine dyes Methylene Blue and Methylene Green were used as electrochemical markers for the detection of sulfonamide and anthracycline preparations able to interact with DNA. The biosensor signal related to HRP oxidation of the markers depends on the relation between their bonded and readily oxidized forms which depends on the nature and concentration of pharmaceuticals. Sulfonamides diminish surface concentration of MB accessible for HRP reaction whereas anthracyclines release intercalated marker and increase the signal. The DNA-HRP sensor developed makes it possible to detect down to 0.002 nmol L-1 of sulfamethoxazole, 0.1 nmol L-1 of sulfadiazine, 0.01 nmol L-1 of sulfamethazine, 0.1 nmol L -1 of sulfaguanine, 0.05 μmol L-1 of rubomycin and 0.08 μmol L-1 of doxorubicin. © 2005 by MDPI.
dc.relation.ispartofseries Sensors
dc.subject Affinity biosensor
dc.subject Anthracycline determination
dc.subject DNA sensor
dc.subject Horseradish peroxidase
dc.subject Sulfonamide determination
dc.title Amperometric DNA-peroxidase sensor for the detection of pharmaceutical preparations
dc.type Article
dc.relation.ispartofseries-issue 6-10
dc.relation.ispartofseries-volume 5
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 364
dc.source.id SCOPUS14243210-2005-5-610-SID34247885997


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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