Kazan Federal University Digital Repository

Proteinases from Bacillus intermedius secreted in the late stages of sporulation

Show simple item record

dc.contributor.author Balaban N.
dc.contributor.author Gabdrakhmanova L.
dc.contributor.author Sharipova M.
dc.contributor.author Mardanova A.
dc.contributor.author Sokolova E.
dc.contributor.author Rudenskaya G.
dc.contributor.author Leshchinskaya I.
dc.date.accessioned 2018-09-17T21:30:57Z
dc.date.available 2018-09-17T21:30:57Z
dc.date.issued 2002
dc.identifier.issn 1234-1010
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135091
dc.description.abstract Background: Proteinases are widely used in various fields of medicine, such as the treatment of burns, purulent wounds, or decubitus ulcers. On the basis of new microbial proteinases produced by nonpathogenic organisms, a new generation of medical preparations can be developed. Representatives of the Bacillus genera are nonpathogenic and are suitable for producing various proteases in large quantities. B. intermedius is shown to produce a set of alkaline proteases at the early and late stationary phase of growth. Material/Methods: The activity of alkaline proteinases was determined using synthetic chromogenous substrates Z-Glu-pNA and Z-Ala-Ala-Leu-pNA. To determine β-galactosidase activity, 2-nitro-β-D-galactopyranosid was used. Spores were calculated by phase-contrast microscopy. Results: During the late stages of sporulation B. intermedius 3-19 cells were shown to secrete two proteinases into the medium: glutamyl endopeptidase, with maximum activity at 40 hours of growth, and subtilisin, with maximum activity at 44 hours of growth. Evidence for the secretion of these enzymes into the medium was provided by measuring β-galactosidase activity. Conclusion: Our results show that proteinases from B. intermedius (glutamyl endopeptidase 2 and subtilisin 2) in the late stationary phase of growth are secreted enzymes. This suggests that these enzymes play a role in sporulation.
dc.relation.ispartofseries Medical Science Monitor
dc.subject Basillus intermedius
dc.subject Glutamyl endopeptidase
dc.subject Secretion
dc.subject Sporulation
dc.subject Thiol-dependent proteinase
dc.title Proteinases from Bacillus intermedius secreted in the late stages of sporulation
dc.type Article
dc.relation.ispartofseries-issue 5
dc.relation.ispartofseries-volume 8
dc.collection Публикации сотрудников КФУ
dc.relation.startpage BR168
dc.source.id SCOPUS12341010-2002-8-5-SID0036276484


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics