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dc.contributor.author | Ilinskaya O. | |
dc.contributor.author | Dreyer F. | |
dc.contributor.author | Mitkevich V. | |
dc.contributor.author | Shaw K. | |
dc.contributor.author | Nick Pace C. | |
dc.contributor.author | Makarov A. | |
dc.date.accessioned | 2018-09-17T20:57:57Z | |
dc.date.available | 2018-09-17T20:57:57Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0961-8368 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/134327 | |
dc.description.abstract | Ribonuclease Sa (pI = 3.5) from Streptomyces aureofaciens and its 3K (D1K, D17K, E41K) (pI = 6.4) and 5K (3K + D25K, E74K) (pI = 10.2) mutants were tested for cytotoxicity. The 5K mutant was cytotoxic to normal and v-ras-transformed NIH3T3 mouse fibroblasts, but RNase Sa and 3K were not. The structure, stability, and activity of the three proteins are comparable, but the net charge at pH 7 increases from -7 for RNase Sa to -1 for 3K and to +3 for 5K. These results suggest that a net positive charge is a key determinant of ribonuclease cytotoxicity. The cytotoxic 5K mutant preferentially attacks v-ras-NIH3T3 fibroblasts, suggesting that mammalian cells expressing the ras-oncogene are potential targets for ribonuclease-based drugs. | |
dc.relation.ispartofseries | Protein Science | |
dc.subject | Charge reversal mutants | |
dc.subject | Cytotoxicity | |
dc.subject | Net charge | |
dc.subject | Ribonuclease Sa | |
dc.subject | v-ras-transformed fibroblasts | |
dc.title | Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 10 | |
dc.relation.ispartofseries-volume | 11 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 2522 | |
dc.source.id | SCOPUS09618368-2002-11-10-SID0036784623 |