dc.contributor.author |
Ilinskaya O. |
|
dc.contributor.author |
Dreyer F. |
|
dc.contributor.author |
Mitkevich V. |
|
dc.contributor.author |
Shaw K. |
|
dc.contributor.author |
Nick Pace C. |
|
dc.contributor.author |
Makarov A. |
|
dc.date.accessioned |
2018-09-17T20:57:57Z |
|
dc.date.available |
2018-09-17T20:57:57Z |
|
dc.date.issued |
2002 |
|
dc.identifier.issn |
0961-8368 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/134327 |
|
dc.description.abstract |
Ribonuclease Sa (pI = 3.5) from Streptomyces aureofaciens and its 3K (D1K, D17K, E41K) (pI = 6.4) and 5K (3K + D25K, E74K) (pI = 10.2) mutants were tested for cytotoxicity. The 5K mutant was cytotoxic to normal and v-ras-transformed NIH3T3 mouse fibroblasts, but RNase Sa and 3K were not. The structure, stability, and activity of the three proteins are comparable, but the net charge at pH 7 increases from -7 for RNase Sa to -1 for 3K and to +3 for 5K. These results suggest that a net positive charge is a key determinant of ribonuclease cytotoxicity. The cytotoxic 5K mutant preferentially attacks v-ras-NIH3T3 fibroblasts, suggesting that mammalian cells expressing the ras-oncogene are potential targets for ribonuclease-based drugs. |
|
dc.relation.ispartofseries |
Protein Science |
|
dc.subject |
Charge reversal mutants |
|
dc.subject |
Cytotoxicity |
|
dc.subject |
Net charge |
|
dc.subject |
Ribonuclease Sa |
|
dc.subject |
v-ras-transformed fibroblasts |
|
dc.title |
Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
10 |
|
dc.relation.ispartofseries-volume |
11 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
2522 |
|
dc.source.id |
SCOPUS09618368-2002-11-10-SID0036784623 |
|