dc.contributor.author |
Vinter V. |
|
dc.contributor.author |
Nevzorova T. |
|
dc.contributor.author |
Konovalova O. |
|
dc.contributor.author |
Salakhov M. |
|
dc.date.accessioned |
2018-09-17T20:48:56Z |
|
dc.date.available |
2018-09-17T20:48:56Z |
|
dc.date.issued |
2005 |
|
dc.identifier.issn |
0869-5652 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/134097 |
|
dc.description.abstract |
Interaction of DNA-hydrolyzing antibodies and DNA with super-twisted plasmid DNA pBR-322 was studied at one of wide spread human autoimmune disease of unknown etiology - exanthematous systemic lupus. It is shown that investigated antibodies and DNA are endonucleases and make one-thread breaks in super-twisted DNA molecules, transforming them into the ring molecules. Using atomic force microscopy, formation of stable immune complex antibody-DNA was registered, the complex size exceeding individual sizes of antibodies and DNA molecule. Probably, abzymes interact with DNA initially with the mechanisms characteristic for antigen-antibody immune complex formation, and further the antibody fermentative properties manifest. However, unlike ordinary DNAses, after phosphodiester bond hydrolysis the antibody molecule is not disengaged from DNA molecule. |
|
dc.relation.ispartofseries |
Doklady Akademii Nauk |
|
dc.title |
Atomic force microscope for investigation of DNA-hydrolyzing activity of anti-DNA antibodies |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
3 |
|
dc.relation.ispartofseries-volume |
405 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
409 |
|
dc.source.id |
SCOPUS08695652-2005-405-3-SID33644629786 |
|