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Noncompetitive immunochemical determination of ribonuclease using transition metal ions and the effect of catalytic hydrogen release | Nekonkurentnoe immunokhimicheskoe opredelenie ribonukleazy s ispol'zovaniem ionov perekhodnykh metallov i éffekta kataliticheskogo vydeleniia vodoroda.

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dc.contributor.author Dykhal I.
dc.contributor.author Mediantseva E.
dc.contributor.author Murtazina N.
dc.contributor.author Safina G.
dc.contributor.author Budnikov G.
dc.contributor.author Kalacheva N.
dc.date.accessioned 2018-09-17T20:45:54Z
dc.date.available 2018-09-17T20:45:54Z
dc.date.issued 2003
dc.identifier.issn 0555-1099
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/134013
dc.description.abstract A noncompetitive variant of immunochemical ribonuclease (RNase) determination has been developed, involving the use of Co(II) as a label. A variety of approaches to labeling the immunological reagent with the metal have been assessed. In the variant proposed, catalytic hydrogen release was used as a means of detecting the label, the amount of which was proportional to RNase concentration. Conditions making it possible to record catalytic hydrogen release fluxes were determined. In the presence of RNase, the electrocatalytic effect was maximum at a concentration of Co(II) in the ammoniac buffer, equal to 2 x 10(-4) M (pH 10.0). The dependence was linear in the range 4-2000 ng/ml RNase concentrations (threshold concentration, 2 ng/ml).
dc.relation.ispartofseries Prikladnaia biokhimiia i mikrobiologiia
dc.title Noncompetitive immunochemical determination of ribonuclease using transition metal ions and the effect of catalytic hydrogen release | Nekonkurentnoe immunokhimicheskoe opredelenie ribonukleazy s ispol'zovaniem ionov perekhodnykh metallov i éffekta kataliticheskogo vydeleniia vodoroda.
dc.type Article
dc.relation.ispartofseries-issue 6
dc.relation.ispartofseries-volume 39
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 630
dc.source.id SCOPUS05551099-2003-39-6-SID2542504743


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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