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dc.contributor.author | Filimonova M. | |
dc.contributor.author | Garusov A. | |
dc.contributor.author | Smetanina T. | |
dc.contributor.author | Andreeva M. | |
dc.contributor.author | Bogomol'naya L. | |
dc.contributor.author | Leshchinskaya I. | |
dc.date.accessioned | 2018-09-17T20:40:51Z | |
dc.date.available | 2018-09-17T20:40:51Z | |
dc.date.issued | 1996 | |
dc.identifier.issn | 0320-9725 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/133873 | |
dc.description.abstract | Comparative analysis of the specificity of Serratia marcescens nuclease isoforms has been carried out. Mononucleotides separated by anion-exchange chromatography with presence of 7 M urea from partially hydrolyzed RNA were identified by reversed-phase HPLC. Both enzymes were found to split phosphodiester bonds at nearly all bases in the nucleic acid chain. However nuclease Sm1 demonstrated a preferred cleavage of phosphodiester bonds near uracil and nuclease Sm2 - near guanine. A possible role of N-terminal tripeptide fragment in nucleases mechanism is discussed. | |
dc.relation.ispartofseries | Biokhimiya | |
dc.title | Isoforms of Serratia marcescens nuclease. Comparative analysis of the substrate specificity | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 10 | |
dc.relation.ispartofseries-volume | 61 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 1800 | |
dc.source.id | SCOPUS03209725-1996-61-10-SID0030251527 |