dc.contributor.author |
Filimonova M. |
|
dc.contributor.author |
Garusov A. |
|
dc.contributor.author |
Smetanina T. |
|
dc.contributor.author |
Andreeva M. |
|
dc.contributor.author |
Bogomol'naya L. |
|
dc.contributor.author |
Leshchinskaya I. |
|
dc.date.accessioned |
2018-09-17T20:40:51Z |
|
dc.date.available |
2018-09-17T20:40:51Z |
|
dc.date.issued |
1996 |
|
dc.identifier.issn |
0320-9725 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/133873 |
|
dc.description.abstract |
Comparative analysis of the specificity of Serratia marcescens nuclease isoforms has been carried out. Mononucleotides separated by anion-exchange chromatography with presence of 7 M urea from partially hydrolyzed RNA were identified by reversed-phase HPLC. Both enzymes were found to split phosphodiester bonds at nearly all bases in the nucleic acid chain. However nuclease Sm1 demonstrated a preferred cleavage of phosphodiester bonds near uracil and nuclease Sm2 - near guanine. A possible role of N-terminal tripeptide fragment in nucleases mechanism is discussed. |
|
dc.relation.ispartofseries |
Biokhimiya |
|
dc.title |
Isoforms of Serratia marcescens nuclease. Comparative analysis of the substrate specificity |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
10 |
|
dc.relation.ispartofseries-volume |
61 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
1800 |
|
dc.source.id |
SCOPUS03209725-1996-61-10-SID0030251527 |
|