Isoforms of Serratia marcescens nuclease. Comparative analysis of the substrate specificity

dc.contributor.author	Filimonova M.
dc.contributor.author	Garusov A.
dc.contributor.author	Smetanina T.
dc.contributor.author	Andreeva M.
dc.contributor.author	Bogomol'naya L.
dc.contributor.author	Leshchinskaya I.
dc.date.accessioned	2018-09-17T20:40:51Z
dc.date.available	2018-09-17T20:40:51Z
dc.date.issued	1996
dc.identifier.issn	0320-9725
dc.identifier.uri	https://dspace.kpfu.ru/xmlui/handle/net/133873
dc.description.abstract	Comparative analysis of the specificity of Serratia marcescens nuclease isoforms has been carried out. Mononucleotides separated by anion-exchange chromatography with presence of 7 M urea from partially hydrolyzed RNA were identified by reversed-phase HPLC. Both enzymes were found to split phosphodiester bonds at nearly all bases in the nucleic acid chain. However nuclease Sm1 demonstrated a preferred cleavage of phosphodiester bonds near uracil and nuclease Sm2 - near guanine. A possible role of N-terminal tripeptide fragment in nucleases mechanism is discussed.
dc.relation.ispartofseries	Biokhimiya
dc.title	Isoforms of Serratia marcescens nuclease. Comparative analysis of the substrate specificity
dc.type	Article
dc.relation.ispartofseries-issue	10
dc.relation.ispartofseries-volume	61
dc.collection	Публикации сотрудников КФУ
dc.relation.startpage	1800
dc.source.id	SCOPUS03209725-1996-61-10-SID0030251527

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Публикации сотрудников КФУ Scopus [24551]
Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.