dc.contributor.author |
Scholz S. |
|
dc.contributor.author |
Korn C. |
|
dc.contributor.author |
Gimadutdinow O. |
|
dc.contributor.author |
Knoblauch M. |
|
dc.contributor.author |
Pingoud A. |
|
dc.contributor.author |
Meiss G. |
|
dc.date.accessioned |
2018-09-17T20:40:11Z |
|
dc.date.available |
2018-09-17T20:40:11Z |
|
dc.date.issued |
2002 |
|
dc.identifier.issn |
0305-1048 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/133858 |
|
dc.description.abstract |
We show here that co-expression of murine CAD with either ICAD-L or ICAD-S in Escherichia coli as well as mammalian cells leads to a functional DFF complex, which after caspase-3 activation releases a nucleolytically active DNase. The chaperone activity of ICAD-S is between one and two orders of magnitude less effective than that of ICAD-L, as deduced from cleavage experiments with different activated recombinant DFF complexes produced in Ecoli. With nucleolytically active EGFP fusion proteins of CAD it is demonstrated that co-expression of ICAD-S, which lacks the C-terminal domain of ICAD-L, including the NLS, leads to a homogeneous intracellular distribution of the DNase in transfected cells, whereas co-expression of human or murine ICAD-L variants lacking the NLS leads to exclusion of EGFP-CAD from the nuclei in ∼50% of cells. These results attribute a particular importance of the NLS in the long isoform of the inhibitor of CAD for nuclear accumulation of the DFF complex in living cells. It is concluded that ICAD-L and ICAD-S in vivo might function as tissue-specific modulators in the regulation of apoptotic DNA degradation by controlling not only the enzymatic activity but also the amount of CAD available in the nuclei of mammalian cells. |
|
dc.relation.ispartofseries |
Nucleic Acids Research |
|
dc.title |
The effect of ICAD-S on the formation and intracellular distribution of a nucleolytically active caspase-activated DNase |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
14 |
|
dc.relation.ispartofseries-volume |
30 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
3045 |
|
dc.source.id |
SCOPUS03051048-2002-30-14-SID0037100613 |
|