Water self-diffusion behavior in yeast cells studied by pulsed field gradient NMR

Abstract

The water self-diffusion behavior in yeast cell water suspension was investigated by pulsed field gradient NMR techniques. Three types of water were detected, which differ according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. Intracellular and extracellular water self-diffusion was restricted; the sizes of restriction regions were approximately 3 and 15-20 μm, respectively. The smallest restriction size was determined as inner cell size. This size and also cell permeability varied with the growth phase of yeast cell. Cell size increased, but permeability decreased with increasing growth time. The values of cell permeabilities P1 d obtained from time dependence of water self-diffusion coefficient were in good agreement with the permeabilities obtained from the exchange rate constants P1 eff. The values of P1 eff were 7×10-6, 1.2×10-6 and 1.6×10-6 m/s, and P1 d were 6.3×10-6, 8.4×10-7, 1.5×10-6 m/s for yeast cells incubated for 9 h (exponential growth phase), 24 h (end of exponential growth phase), and 48 h (stationary growth phase), respectively. © 2003 Elsevier Science B.V. All rights reserved.