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Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase

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dc.contributor.author Chastukhina I.
dc.contributor.author Sharipova M.
dc.contributor.author Gabdrakhmanova L.
dc.contributor.author Balaban N.
dc.contributor.author Kostrov S.
dc.contributor.author Rudenskaya G.
dc.contributor.author Leshchinskaya I.
dc.date.accessioned 2018-09-17T20:31:24Z
dc.date.available 2018-09-17T20:31:24Z
dc.date.issued 2005
dc.identifier.issn 0026-2617
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133612
dc.description.abstract We studied the biosynthesis of bacillus intermedius glutamyl endopeptidase in the recombinant bacillus subtilis strain AJ73 Δ58.21 during the stationary growth phase. We optimized the composition of the culture medium to favor effective enzyme production during the stationary growth phase and found that the nutritional requirements for glutamyl endopeptidase synthesis were different in the stationary phase and the growth retardation phase. Proteinase accumulation was activated by complex organic substrates (casein and gelatin). During the final stages of the culture growth, the enzyme production was stimulated by Ca2+, Mn2+, and Co2+ and inhibited by Zn2+, Fe2+, and Cu2+. The synthesis of glutamyl endopeptidase in the late stationary phase was not inhibited by glucose, unlike that in the trophophase during proliferation. We conclude that the regulatory mechanisms of proteinase synthesis during vegetative growth and sporulation are different. © 2005 Pleiades Publishing, Inc.
dc.relation.ispartofseries Microbiology
dc.subject Glutamyl endopeptidase
dc.subject Growth conditions
dc.subject Recombinant strain
dc.subject Regulation of biosynthesis
dc.subject Sporulation
dc.title Peculiarities of the biosynthesis of Bacillus intermedius glutamyl endopeptidase in recombinant Bacillus subtilis cells during the stationary growth phase
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 74
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 32
dc.source.id SCOPUS00262617-2005-74-1-SID17444361964


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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