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Analysis of the Ca2+ response of mycelial fungi to external effects by the recombinant aequorin method

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dc.contributor.author Kozlova O.
dc.contributor.author Egorov S.
dc.contributor.author Kupriyanova-Ashina F.
dc.contributor.author Rid N.
dc.contributor.author El'-Registan G.
dc.date.accessioned 2018-09-17T20:31:19Z
dc.date.available 2018-09-17T20:31:19Z
dc.date.issued 2004
dc.identifier.issn 0026-2617
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133610
dc.description.abstract Using the mutant strain Aspergillus awamori 66A, producing the recombinant Ca2+ -dependent photosensitive protein aequorin, the dynamics of Ca2+ was studied for the first time in the cytosol of micromycetes exposed to stressful factors, such as an increase in extracellular Ca 2+ to 50 mM, hypoosmotic shock, and mechanical shock. The cell response to stress proved to involve an increase in the Ca2+ concentration in the cytosol, which was determined from the amplitude of aequorin luminescence and the time of the amplitude enhancement and relaxation. The level of the Ca2+ response depended on the physiological stimulus. Inhibitory analysis with various agents that block Ca2+ channels and with agonists that specifically enhance the activity of the channels suggested that (1) the level of Ca2+ in the cytosol of micromycetes increases in response to stress because of the ion influx from both the growth medium and intracellular reservoirs and (2) potential-dependent transport systems play the major role in the Ca2+ influx into the cytosol of the micromycete cells. © 2004 MAIK "Nauka/ Interperiodica".
dc.relation.ispartofseries Microbiology
dc.subject Aspergillus awamori
dc.subject Ca 2+ dynamics
dc.subject Mycelial fungi
dc.subject Recombinant aequorin
dc.subject Stress
dc.title Analysis of the Ca2+ response of mycelial fungi to external effects by the recombinant aequorin method
dc.type Article
dc.relation.ispartofseries-issue 6
dc.relation.ispartofseries-volume 73
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 629
dc.source.id SCOPUS00262617-2004-73-6-SID15044354777

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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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