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The effect of thermal treatment on the catalytic activity and biological properties of Bacillus intermedius ribonuclease

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dc.contributor.author Kolpakov A.
dc.contributor.author Il'Inskaya O.
dc.contributor.author Ivanchenko O.
dc.contributor.author Karamova N.
dc.date.accessioned 2018-09-17T20:30:52Z
dc.date.available 2018-09-17T20:30:52Z
dc.date.issued 2001
dc.identifier.issn 0026-2617
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133597
dc.description.abstract Bacillus intermedius ribonuclease (binase), which is known to exert a growth-stimulating effect at low concentrations and a genotoxic effect at high concentrations, loses these abilities completely after exposure to 100°C for 10 min, but retains approximately 95% of its catalytic activity and structural integrity. Other types of modification, such as photoinactivation and site-specific mutagenesis, gave rise to enzyme forms with unaltered structure but reduced (sometimes to trace amounts) catalytic activity. Genotoxicity was always proportional to the catalytic activity of the native enzyme, while a notable growth-stimulating effect could be exerted by enzymes with low activity. The loss of biological activity of thermoinactivated binase was related to the increase in the number of negatively charged groups on the enzyme surface, which led to a substantial decline in the adhesive properties of the enzyme. © 2001 MAIK "Nauka/Interperiodica".
dc.relation.ispartofseries Microbiology
dc.subject Adsorption
dc.subject Bacillus intermedius ribonuclease
dc.subject Catalytic activity
dc.subject Genotoxicity
dc.subject Growth stimulation
dc.subject Thermoinactivation
dc.title The effect of thermal treatment on the catalytic activity and biological properties of Bacillus intermedius ribonuclease
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 70
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 18
dc.source.id SCOPUS00262617-2001-70-1-SID27844559298


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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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