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Stabilization of enzymes by dormancy autoinducers as a possible mechanism of resistance of resting microbial forms

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dc.contributor.author Kolpakov A.
dc.contributor.author Il'inskaya O.
dc.contributor.author Bespalov M.
dc.contributor.author Kupriyanova-Ashina F.
dc.contributor.author Gal'chenko V.
dc.contributor.author Kurganov B.
dc.contributor.author El'-Registan G.
dc.date.accessioned 2018-09-17T20:30:29Z
dc.date.available 2018-09-17T20:30:29Z
dc.date.issued 2000
dc.identifier.issn 0026-2617
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133589
dc.description.abstract Alkyl-substituted hydroxybenzenes (AHBs), autoinducers of microbial dormancy (or d1 factors), were found to stabilize the structure of protein macromolecules, making them metabolically less active and more resistant to stresses. In vitro experiments with the Bacillus intermedius ribonuclease and chymotrypsin showed that the degree of the physical and chemical stability of these enzymes treated with AHBs depends on their concentration and incubation time. Experiments with RNase, which is capable of refolding, i.e., renaturation after heat denaturation, revealed that AHBs efficiently interact with both intact and denatured proteins. The data obtained allow the inference to be made that d1 factors may play the role of natural chemical chaperons, blocking metabolism in dormant cells through the formation of catalytically inactive thermostable complexes with enzymes. © 2000 MAIK "Nauka/Interperiodica".
dc.relation.ispartofseries Microbiology
dc.subject Alkyl-substituted hydroxybenzenes
dc.subject Chemical chaperons
dc.subject D1 factors of microorganisms
dc.subject Dormancy
dc.subject Dormancy autoinducers
dc.subject Metabolic block
dc.subject Thermostability of enzymes
dc.title Stabilization of enzymes by dormancy autoinducers as a possible mechanism of resistance of resting microbial forms
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 69
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 180
dc.source.id SCOPUS00262617-2000-69-2-SID0037736774

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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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