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dc.contributor.author | Ivanchenko O. | |
dc.contributor.author | Il'Inskaya O. | |
dc.contributor.author | Karamova N. | |
dc.contributor.author | Kipenskaya L. | |
dc.contributor.author | Leshchinskaya I. | |
dc.date.accessioned | 2018-09-17T20:28:18Z | |
dc.date.available | 2018-09-17T20:28:18Z | |
dc.date.issued | 1997 | |
dc.identifier.issn | 0026-3656 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/133532 | |
dc.description.abstract | This paper deals with the induction of the SOS-functions of a bacterial cell by the exogenous ribonuclease of Bacillus intermedius. Two test systems were employed, which made it possible to quantitatively estimate SOS-response induction activity in gram-positive and gram-negative bacteria. It was established that the catalytically active enzyme elicits the SOS-response in the cells of Escherichia coli PQ 37 and causes prophage induction in the lysogenic strain Bacillus subtilis 168 cplOS WT (a phenomenon related to the SOS-response). An enzyme with a His101Glu-substituted active center, exhibiting residual catalytic activity, failed to induce the SOS-response in an SOS-chromotest with E. coli PQ 37, indicating dependence of the SOSinducing effect of the enzyme on its catalytic activity. It is suggested that the effect of active ribonuclease on membrane-bound and cytoplasmic RNA causes a change in the nucleotide pool and, as a consequence, elicits the SOS-response of the cell. | |
dc.relation.ispartofseries | Mikrobiologiya | |
dc.subject | Bacillus intermedius | |
dc.subject | Microbial test systems | |
dc.subject | Ribonuclease | |
dc.subject | SOS-response | |
dc.title | Induction of the SOS-response in test bacteria by bacillus intermedius RNase | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 4 | |
dc.relation.ispartofseries-volume | 66 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 444 | |
dc.source.id | SCOPUS00263656-1997-66-4-SID0031180044 |