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Biosynthesis of endochitinase of Serratia marcescens

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dc.contributor.author Gabdrakhmanova L.
dc.contributor.author Petukhova E.
dc.contributor.author Sokolova R.
dc.contributor.author Yusupova D.
dc.date.accessioned 2018-09-17T20:28:05Z
dc.date.available 2018-09-17T20:28:05Z
dc.date.issued 1998
dc.identifier.issn 0026-2633
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133526
dc.description.abstract The growth of a mutant strain of Serratia marcescens with high chitinase activity and the biosynthesis of endochitinase by this strain were investigated. The study was carried out using semisynthetic culture medium without inducers and culture medium containing colloidal chitin as a sole nitrogen and carbon source, with and without mitomycin C. The mutant strain, unlike the native one, was shown to produce endochitinase and to secrete the enzyme into the medium during the growth on culture medium without the inducers, chitin and mitomycin C. During growth on the medium with chitin the mutant strain differed from the native one with a short lag-phase of growth, the early appearance of endochitinase in the culture liquid and a high level of endochitinase activity. The difference between the strains disappeared after the addition of mitomycin C, an inducer of the cell SOS-response, to the culture medium containing chitin. Specific endochitinase activity of S. marcescens mutant strain grown on various culture media had two maxima, namely at the beginning and at the end of the stationary phase. Mitomycin C increased the specific activity in a second peak of endochitinase activity during the growth of the mutant strain.
dc.relation.ispartofseries Microbios
dc.subject Biosynthesis
dc.subject Endochitinase
dc.subject Mitomycin C
dc.subject Serratia marcescens
dc.title Biosynthesis of endochitinase of Serratia marcescens
dc.type Article
dc.relation.ispartofseries-issue 385
dc.relation.ispartofseries-volume 96
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 157
dc.source.id SCOPUS00262633-1998-96-385-SID0032242966


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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