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Structural and functional characterization of mitochondrial EndoG, a sugar non-specific nuclease which plays an important role during apoptosis

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dc.contributor.author Schäfer P.
dc.contributor.author Scholz S.
dc.contributor.author Gimadutdinow O.
dc.contributor.author Cymerman I.
dc.contributor.author Bujnicki J.
dc.contributor.author Ruiz-Carrillo A.
dc.contributor.author Pingoud A.
dc.contributor.author Meiss G.
dc.date.accessioned 2018-09-17T20:22:47Z
dc.date.available 2018-09-17T20:22:47Z
dc.date.issued 2004
dc.identifier.issn 0022-2836
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133423
dc.description.abstract Combining sequence analysis, structure prediction, and site-directed mutagenesis, we have investigated the mechanism of catalysis and substrate binding by the apoptotic mitochondrial nuclease EndoG, which belongs to the large family of DNA/RNA non-specific ββα-Me-finger nucleases. Catalysis of phosphodiester bond cleavage involves several highly conserved amino acid residues, namely His143, Asn174, and Glu182 required for water activation and metal ion binding, as well as Arg141 required for proper substrate binding and positioning, respectively. These results indicate that EndoG basically follows a similar mechanism as the Serratia nuclease, the best studied representative of the family of DNA/RNA non-specific nucleases, but that differences are observed for transition state stabilisation. In addition, we have identified two putative DNA/RNA binding residues of bovine EndoG, Arg135 and Arg186, strictly conserved only among mammalian members of the nuclease family, suggesting a similar mode of binding to single and double-stranded nucleic acid substrates by these enzymes. Finally, we demonstrate by ectopic expression of active and inactive variants of bovine EndoG in HeLa and CV1-cells that extramitochondrial active EndoG by itself induces cell death, whereas expression of an enzymatically inactive variant does not. © 2004 Elsevier Ltd. All rights reserved.
dc.relation.ispartofseries Journal of Molecular Biology
dc.subject Active-site
dc.subject AIF, apoptosis inducing factor
dc.subject Apoptosis
dc.subject Catalysis
dc.subject EndoG, mitochondrial endonuclease G
dc.subject Endonuclease G
dc.subject HSV-1, herpes simplex type-1 virus
dc.subject Nuclease
dc.subject tEndoG, truncated bovine EndoG
dc.subject WAH-1, worm-AIF-homolog 1
dc.title Structural and functional characterization of mitochondrial EndoG, a sugar non-specific nuclease which plays an important role during apoptosis
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 338
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 217
dc.source.id SCOPUS00222836-2004-338-2-SID1842452746

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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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