Электронный архив

Contribution of arginine-82 and arginine-86 to catalysis of RNases from Bacillus intermedius (binase)

Показать сокращенную информацию

dc.contributor.author Yakovlev G.
dc.contributor.author Struminskaya N.
dc.contributor.author Znamenskaya L.
dc.contributor.author Kipenskaya L.
dc.contributor.author Leschinskaya I.
dc.contributor.author Hartley R.
dc.date.accessioned 2018-09-17T20:12:53Z
dc.date.available 2018-09-17T20:12:53Z
dc.date.issued 1998
dc.identifier.issn 0014-5793
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133191
dc.description.abstract To elucidate the functional role of Arg82 and Arg86 in the enzyme activity of binase, the extracellular ribonuclease of Bacillus intermedias, we used site-directed mutagenesis. On cleavage of various substrates the catalytic activity of binase mutant Arg86Ala is 2.7 x 103-7.7 X 103 times less than that of the native enzyme. The decrease in activity is determined preferentially by the decrease in the molecular rate constant k(cat) with a relatively small change of enzyme-substrate affinity, characterized by K(m). This is the expected result if Arg86 acts to lower the energy of a transition state of the reaction. The replacement of Arg82 by Ala causes a 519-fold activity decrease, depending on the substrate. We propose that this residue does not have a direct catalytic function in the molecular mechanism of the nase action and that the activity decrease of binase on the replacement of Arg82 by alanine is mediated by the effect of Arg82 on the pK of catalytic residues.
dc.relation.ispartofseries FEBS Letters
dc.subject Catalytic property
dc.subject Ribonuclease
dc.subject Site-directed mutagenesis
dc.title Contribution of arginine-82 and arginine-86 to catalysis of RNases from Bacillus intermedius (binase)
dc.type Article
dc.relation.ispartofseries-issue 1-2
dc.relation.ispartofseries-volume 428
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 57
dc.source.id SCOPUS00145793-1998-428-12-SID0032557408


Файлы в этом документе

Данный элемент включен в следующие коллекции

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Показать сокращенную информацию

Поиск в электронном архиве


Расширенный поиск

Просмотр

Моя учетная запись

Статистика