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Isolation and characterization of glutamyl endopeptidase 2 from Bacillus intermedius 3-19

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dc.contributor.author Balaban N.
dc.contributor.author Mardanova A.
dc.contributor.author Sharipova M.
dc.contributor.author Gabdrakhmanova L.
dc.contributor.author Sokolova E.
dc.contributor.author Garusov A.
dc.contributor.author Milgotina E.
dc.contributor.author Rudenskaya G.
dc.contributor.author Leshchinskaya I.
dc.date.accessioned 2018-09-17T20:09:06Z
dc.date.available 2018-09-17T20:09:06Z
dc.date.issued 2003
dc.identifier.issn 0006-2979
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133104
dc.description.abstract The culture filtrate of Bacillus intermedius 3-19 was used for isolation by chromatography on CM-cellulose and Mono S columns of a proteinase that is secreted during the late stages of growth. The enzyme is irreversibly inhibited by the inhibitor of serine proteinases diisopropyl fluorophosphate, has two pH optima (7.2 and 9.5) for casein hydrolysis and one at pH 8.5 for Z-Glu-pNA hydrolysis. The molecular weight of the enzyme is 26.5 kD. The Km for Z-Glu-pNA hydrolysis is 0.5 mM. The temperature and pH dependences of the stability of the proteinase were studied. The enzyme was identified as glutamyl endopeptidase 2. The N-terminal sequence (10 residues) and amino acid composition of the enzyme were determined. The enzyme hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin.
dc.relation.ispartofseries Biochemistry (Moscow)
dc.subject Bacillus intermedius
dc.subject Glutamyl endopeptidase
dc.subject Isolation
dc.subject Properties
dc.subject Proteinase
dc.title Isolation and characterization of glutamyl endopeptidase 2 from Bacillus intermedius 3-19
dc.type Article
dc.relation.ispartofseries-issue 11
dc.relation.ispartofseries-volume 68
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1217
dc.source.id SCOPUS00062979-2003-68-11-SID0742268906


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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