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Study of the mechanism of action of p-chloromercuribenzoate on endonuclease from the bacterium Serratia marcescens

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dc.contributor.author Filimonova M.
dc.contributor.author Gubskaya V.
dc.contributor.author Nuretdinov I.
dc.contributor.author Benedik M.
dc.contributor.author Cherepanova N.
dc.contributor.author Leshchinskaya I.
dc.date.accessioned 2018-09-17T20:08:54Z
dc.date.available 2018-09-17T20:08:54Z
dc.date.issued 2001
dc.identifier.issn 0006-2979
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133100
dc.description.abstract The mechanism of action of p-chloromercuribenzoate (PCMB) on Serratia marcescens nuclease was investigated. The analysis showed that PCMB forms complexes with DNA. Binding of C7H5O2Hg+ to DNA changes the secondary structure of the DNA. These changes alter the enzymatic activity of S. marcescens nuclease, which was previously found to be sensitive to the secondary structure of the substrates. The nuclease activity was either suppressed or stimulated in the presence of PCMB depending on the C7H5O2Hg+ to nucleotide equivalent ratio. Binding of C7H5O2Hg+ to DNA did not form an abortive enzyme-substrate complex. Binding of Mg2+ to the C7H5O2Hg-DNA complex caused appropriate changes in secondary structure of the substrate. Since Mg2+ and C7H5O2Hg+, though differing in the type of metal cation, are similar in their mechanisms of influence on enzymatic activity of S. marcescens nuclease, the identity of other metal-containing effectors in their mechanism of action on Serratia marcescens nuclease is assumed.
dc.relation.ispartofseries Biochemistry (Moscow)
dc.subject CD
dc.subject DNA
dc.subject Endonuclease
dc.subject PCMB
dc.subject Serratia marcescens
dc.title Study of the mechanism of action of p-chloromercuribenzoate on endonuclease from the bacterium Serratia marcescens
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 66
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 323
dc.source.id SCOPUS00062979-2001-66-3-SID0035263333


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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