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Preparation of Spin-Labeled Bacterial Ribonuclease from Bacillus intermedius 7P for EPR Studies of Protein Dynamics

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dc.contributor.author Yagodina L.
dc.contributor.author Altshuler A.
dc.contributor.author Abreimova Y.
dc.date.accessioned 2018-09-17T20:08:52Z
dc.date.available 2018-09-17T20:08:52Z
dc.date.issued 1999
dc.identifier.issn 0006-2979
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133099
dc.description.abstract The optimal conditions for labeling of binase (a bacterial ribonuclease isolated from Bacillus intermedius 7P) with a bromoacetamide spin label have been determined. The label is suitable for probing the dynamics of the protein by electron paramagnetic resonance (EPR). Binase samples specifically labeled at residue His-101 of the active center were prepared by incubation for 48 h at 20°C in potassium phosphate buffer (pH 5.5) containing the bromoacetamide (1:3 protein/label molar ratio). Fluorescence assay showed that the structure of the labeled binase is indistinguishable from that of the native protein.
dc.relation.ispartofseries Biochemistry (Moscow)
dc.subject Binase
dc.subject EPR
dc.subject Fluorescence assay
dc.subject Spin label
dc.title Preparation of Spin-Labeled Bacterial Ribonuclease from Bacillus intermedius 7P for EPR Studies of Protein Dynamics
dc.type Article
dc.relation.ispartofseries-issue 8
dc.relation.ispartofseries-volume 64
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 957
dc.source.id SCOPUS00062979-1999-64-8-SID0033173830


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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