Preparation of Spin-Labeled Bacterial Ribonuclease from Bacillus intermedius 7P for EPR Studies of Protein Dynamics

dc.contributor.author	Yagodina L.
dc.contributor.author	Altshuler A.
dc.contributor.author	Abreimova Y.
dc.date.accessioned	2018-09-17T20:08:52Z
dc.date.available	2018-09-17T20:08:52Z
dc.date.issued	1999
dc.identifier.issn	0006-2979
dc.identifier.uri	https://dspace.kpfu.ru/xmlui/handle/net/133099
dc.description.abstract	The optimal conditions for labeling of binase (a bacterial ribonuclease isolated from Bacillus intermedius 7P) with a bromoacetamide spin label have been determined. The label is suitable for probing the dynamics of the protein by electron paramagnetic resonance (EPR). Binase samples specifically labeled at residue His-101 of the active center were prepared by incubation for 48 h at 20°C in potassium phosphate buffer (pH 5.5) containing the bromoacetamide (1:3 protein/label molar ratio). Fluorescence assay showed that the structure of the labeled binase is indistinguishable from that of the native protein.
dc.relation.ispartofseries	Biochemistry (Moscow)
dc.subject	Binase
dc.subject	EPR
dc.subject	Fluorescence assay
dc.subject	Spin label
dc.title	Preparation of Spin-Labeled Bacterial Ribonuclease from Bacillus intermedius 7P for EPR Studies of Protein Dynamics
dc.type	Article
dc.relation.ispartofseries-issue	8
dc.relation.ispartofseries-volume	64
dc.collection	Публикации сотрудников КФУ
dc.relation.startpage	957
dc.source.id	SCOPUS00062979-1999-64-8-SID0033173830

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Публикации сотрудников КФУ Scopus [24551]
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