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Effects of medium viscosity on kinetics of the enzymatic reaction catalyzed by bacterial RNase

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dc.contributor.author Stupishina E.
dc.contributor.author Vershinina V.
dc.date.accessioned 2018-09-17T20:08:47Z
dc.date.available 2018-09-17T20:08:47Z
dc.date.issued 1998
dc.identifier.issn 0006-2979
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133097
dc.description.abstract Effects of medium viscosity on kinetic parameters of poly(U) hydrolysis catalyzed by RNase from Bac. intermedius 7P (binase) were studied in solutions of sucrose (4-50 wt. %) and glycerol (35-62 wt. %) in Tris-sodium acetate buffer (pH 7.5) at 25°C. The rate constant of reaction kcat was practically unchanged over a wide range of viscosities (1-15 cP for sucrose and 2.5-3 cP for glycerol). In glycerol solutions, kcat slightly increased with viscosity increase from 4 to 10 cP. Addition of NaCl to the buffer medium resulted in an inhibitory effect of Na+ on kcat, prevented by 50% sucrose or 60% glycerol. It is concluded that binase-catalyzed poly(U) cleavage occurs through a "tense"-substrate mechanism, similarly to reactions catalyzed by α-chymotrypsin, trypsin, and laccase.
dc.relation.ispartofseries Biochemistry (Moscow)
dc.subject Enzymatic catalysis
dc.subject Kinetic parameters
dc.subject Medium viscosity
dc.subject RNase from Bac. Intermedius 7P
dc.title Effects of medium viscosity on kinetics of the enzymatic reaction catalyzed by bacterial RNase
dc.type Article
dc.relation.ispartofseries-issue 11
dc.relation.ispartofseries-volume 63
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1277
dc.source.id SCOPUS00062979-1998-63-11-SID0343730197


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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