Kazan Federal University Digital Repository

Effects of P2-purinoceptor antagonists on degradation of adenine nucleotides by ecto-nucleotidases in folliculated oocytes of Xenopus laevis

Show simple item record

dc.contributor.author Ziganshin A.
dc.contributor.author Ziganshina L.
dc.contributor.author King B.
dc.contributor.author Pintor J.
dc.contributor.author Burnstock G.
dc.date.accessioned 2018-09-17T20:08:25Z
dc.date.available 2018-09-17T20:08:25Z
dc.date.issued 1996
dc.identifier.issn 0006-2952
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133087
dc.description.abstract The aim of the present study was to examine the effects of a number of P2-purinoceptor antagonists on degradation of adenine nucleotides by Xenopus laevis oocyte ecto-nucleotidases. Folliculated oocytes readily metabolize all three naturally-occurring nucleotides, the order of preferential substrates being ATP > ADP > AMP. The degradation of ATP and ADP was decreased significantly in the presence of several P2X- and P2Y-purinoceptor antagonists, including suramin, PPADS, Cibacron blue, Coomassie Brilliant blue, Evans blue, Trypan blue, Congo red, and PIT (each compound was used at 100 μM). All these compounds inhibited the degradation of ATP by up to 60%, whereas the hydrolysis of ADP was inhibited by Congo red and PIT by 75-80%. In addition, DIDS (100 μM) and TNP-ATP (100 μM) selectively inhibited the breakdown of ATP, and sodium azide (10 mM) selectively inhibited the breakdown of ADP. The enzymatic breakdown of either ATP or ADP was unaffected by 8-pSPT (100 μM), an antagonist of P1-purinoceptors, or by oxidized ATP (100 μM), an antagonist of P2Z-purinoceptors. The degradation of AMP was prevented completely by PIT (100 μM) and inhibited significantly by Congo red (100 μM). In conclusion, the present study shows that most of currently available antagonists of P2-purinoceptors inhibit the enzymatic breakdown of extracellular ATP and ADP. The inhibitory effect on ecto-nucleotidase activity should be taken into account when these antagonists are used in pharmacological experiments.
dc.relation.ispartofseries Biochemical Pharmacology
dc.subject ecto-nucleotidase
dc.subject P2-purinoceptor antagonists
dc.subject Xenopus oocytes
dc.title Effects of P2-purinoceptor antagonists on degradation of adenine nucleotides by ecto-nucleotidases in folliculated oocytes of Xenopus laevis
dc.type Article
dc.relation.ispartofseries-issue 7
dc.relation.ispartofseries-volume 51
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 897
dc.source.id SCOPUS00062952-1996-51-7-SID0029940581


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics