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Cytotoxicity of RNases is increased by cationization and counteracted by KCa channels

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dc.contributor.author Ilinskaya O.
dc.contributor.author Koschinski A.
dc.contributor.author Mitkevich V.
dc.contributor.author Repp H.
dc.contributor.author Dreyer F.
dc.contributor.author Pace C.
dc.contributor.author Makarov A.
dc.date.accessioned 2018-09-17T20:08:23Z
dc.date.available 2018-09-17T20:08:23Z
dc.date.issued 2004
dc.identifier.issn 0006-291X
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133086
dc.description.abstract KCa channels are involved in control of cell proliferation and differentiation. Here we have revealed their role in overcoming the RNase-induced cytotoxicity. Toxic effects of Streptomyces aureofaciens RNases Sa, Sa2, Sa3, and of RNase Sa charge reversal mutants on the human embryonic kidney cell lines differing only by the presence of KCa channels were characterized. In contrast to other RNases, a basic variant of RNase Sa and RNase Sa3 exhibit significant cytotoxic activity of the same order of magnitude as onconase. Our data indicate the absence of a correlation between catalytic activity and stability of RNases and cytotoxicity. On the other hand, cationization enhances toxic effect of an RNase indicating the major role of a positive charge. Essentially lower sensitivity to cytotoxic microbial RNases of cells expressing KCa channels was found. These results suggest that cells without the KCa channel activity cannot counteract toxic effect of RNases. © 2003 Elsevier Inc. All rights reserved.
dc.relation.ispartofseries Biochemical and Biophysical Research Communications
dc.subject Charge reversal mutants
dc.subject Cytotoxicity
dc.subject Human embryonic kidney cells
dc.subject KCa channels
dc.subject Ribonuclease Sa
dc.title Cytotoxicity of RNases is increased by cationization and counteracted by KCa channels
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 314
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 550
dc.source.id SCOPUS0006291X-2004-314-2-SID0347635412


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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