dc.contributor.author |
Babkina S. |
|
dc.contributor.author |
Medyantseva E. |
|
dc.contributor.author |
Budnikov H. |
|
dc.contributor.author |
Tyshlek M. |
|
dc.date.accessioned |
2018-09-17T20:05:22Z |
|
dc.date.available |
2018-09-17T20:05:22Z |
|
dc.date.issued |
1996 |
|
dc.identifier.issn |
0003-2700 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/133014 |
|
dc.description.abstract |
A method of DNA immobilization on cellulose nitrate films has been developed. Modified films of uniform and stable surface have been used to devise two variants of solid-phase enzyme immunoassays of antibodies. The co-immobilization of enzyme label (cholinesterase) and the DNA molecules makes it possible to carry out the procedure of solid-phase enzyme immunoassay without any separation of components. Thus, it takes only 15 min to diagnose an autoimmune disease (Aleutian disease of minks) with the immunoenzyme amperometric sensor, with a lower detection limit for antibodies of 0.5 × 10-10 M. For scaled diagnosing, solid-phase enzyme immunoassay on DNA-modified films with prior separation of components and spectrophotomeiric registration of peroxidase activity has been developed. The time for determination was 30 min, with a lower detection limit of 7.4 × 10-12 M. |
|
dc.relation.ispartofseries |
Analytical Chemistry |
|
dc.title |
New Variants of Enzyme Immunoassay of Antibodies to DNA |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
21 |
|
dc.relation.ispartofseries-volume |
68 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
3827 |
|
dc.source.id |
SCOPUS00032700-1996-68-21-SID0030292410 |
|