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The specific immunochemical detection of 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid pesticides by amperometric cholinesterase biosensors

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dc.contributor.author Medyantseva E.
dc.contributor.author Vertlib M.
dc.contributor.author Kutyreva M.
dc.contributor.author Khaldeeva E.
dc.contributor.author Budnikov G.
dc.contributor.author Eremin S.
dc.date.accessioned 2018-09-17T20:05:03Z
dc.date.available 2018-09-17T20:05:03Z
dc.date.issued 1997
dc.identifier.issn 0003-2670
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/133006
dc.description.abstract Two specific immunoassays for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) herbicides were developed. These methods are based on a specific immunoreaction of pesticides with antibodies followed by electrochemical detection of unbounded pesticides by its inhibition effect on the amperometric cholinesterase biosensor. The total time of assay is less than 20 min. The antibodies are used in solution or immobilised on the nitrocellulose membrane. The immobilised antibodies could be regenerated several times and are stable for at least 30 days. The dynamic ranges of detection of 2,4-D and 2,4,5-T are 1x10-11-5x10-7 M and 5x10-11-5x10-7 M, respectively. The developed method was applied for 2,4-D determination in milk without sample pre-treatment.
dc.relation.ispartofseries Analytica Chimica Acta
dc.subject 2,4,5-trichlorophenoxyacetic acid
dc.subject 2,4-dichlorophenoxyacetic acid
dc.subject Amperometric cholinesterase biosensor
dc.subject Immunoassay
dc.subject Pesticide
dc.title The specific immunochemical detection of 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid pesticides by amperometric cholinesterase biosensors
dc.type Article
dc.relation.ispartofseries-issue 1-2
dc.relation.ispartofseries-volume 347
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 71
dc.source.id SCOPUS00032670-1997-347-12-SID0030861794


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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