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dc.contributor.author | Evtugyn G. | |
dc.contributor.author | Budnikov H. | |
dc.contributor.author | Nikolskaya E. | |
dc.date.accessioned | 2018-09-17T20:04:59Z | |
dc.date.available | 2018-09-17T20:04:59Z | |
dc.date.issued | 1996 | |
dc.identifier.issn | 0003-2654 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/133004 | |
dc.description.abstract | The influence of non-ionogenic surfactants, i.e., Tween-20, Triton X-100 and PEG-10,000, on the response of cholinesterase-based potentiometric biosensors and their sensitivity towards reversible and irreversible inhibitors were investigated. Acetyl-and butyrylcholinesterases were immobilized on nylon, cellulose nitrate films and tracing paper and were introduced into an assembly of potentiometric biosensors. The effect of surface-active compounds depends on the hydrophilic properties and porosity of the enzyme support material and the inhibition mechanism. In the range 0.002-0.3% m/v the surfactants show a reversible inhibiting effect on biosensor response. At tower concentrations (down to 10-4% m/v) the surfactants alter the analytical characteristics of reversible and irreversible inhibitor determination. The use of surface-active additives improves the biosensor selectivity in multi-component media. | |
dc.relation.ispartofseries | Analyst | |
dc.subject | Cholinesterase biosensor | |
dc.subject | Inhibitor determination | |
dc.subject | Non-ionogenic surfactants | |
dc.title | Influence of surface-active compounds on the response and sensitivity of cholinesterase biosensors for inhibitor determination | |
dc.type | Conference Paper | |
dc.relation.ispartofseries-issue | 12 | |
dc.relation.ispartofseries-volume | 121 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 1911 | |
dc.source.id | SCOPUS00032654-1996-121-12-SID0030480213 |