dc.contributor.author |
Rozov A. |
|
dc.contributor.author |
Jappy D. |
|
dc.date.accessioned |
2018-04-05T07:09:32Z |
|
dc.date.available |
2018-04-05T07:09:32Z |
|
dc.date.issued |
2017 |
|
dc.identifier.issn |
1064-3745 |
|
dc.identifier.uri |
http://dspace.kpfu.ru/xmlui/handle/net/129812 |
|
dc.description.abstract |
© Springer Science+Business Media LLC 2017. A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321–328, 2004). However, in addition to the well-developed repertoire of molecular biological techniques, these types of studies also require electrophysiological methods that allow a wide range of receptor activation protocols that can adequately assess desensitization, inactivation, ion permeability, and other properties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261–287, 1992; Jonas and Sakmann, J Physiol 455:143–171, 1992). |
|
dc.relation.ispartofseries |
Methods in Molecular Biology |
|
dc.subject |
Calcium permeability |
|
dc.subject |
Fast agonist application |
|
dc.subject |
Kinetics |
|
dc.subject |
Magnesium block |
|
dc.subject |
Recombinant NMDA receptors |
|
dc.title |
Functional analysis of recombinant channels in host cells using a fast agonist application system |
|
dc.type |
Chapter |
|
dc.relation.ispartofseries-volume |
1677 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
163 |
|
dc.source.id |
SCOPUS10643745-2017-1677-SID85030786311 |
|