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dc.contributor.author | Rozov A. | |
dc.contributor.author | Jappy D. | |
dc.date.accessioned | 2018-04-05T07:09:32Z | |
dc.date.available | 2018-04-05T07:09:32Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.uri | http://dspace.kpfu.ru/xmlui/handle/net/129812 | |
dc.description.abstract | © Springer Science+Business Media LLC 2017. A reduced recombinant system provides a unique opportunity to study the biophysical properties of NMDAR channels with known subunit compositions, by using a point mutation approach to analyze the structural determinants of receptor function (Wollmuth and Sobolevsky, Trends Neurosci 27:321–328, 2004). However, in addition to the well-developed repertoire of molecular biological techniques, these types of studies also require electrophysiological methods that allow a wide range of receptor activation protocols that can adequately assess desensitization, inactivation, ion permeability, and other properties of the channels. Currently, one of the most well-developed techniques suitable for addressing these issues is use of the fast agonist application system for rapid activation of ligand gated ion-channels (Colquhoun et al., J Physiol 458:261–287, 1992; Jonas and Sakmann, J Physiol 455:143–171, 1992). | |
dc.relation.ispartofseries | Methods in Molecular Biology | |
dc.subject | Calcium permeability | |
dc.subject | Fast agonist application | |
dc.subject | Kinetics | |
dc.subject | Magnesium block | |
dc.subject | Recombinant NMDA receptors | |
dc.title | Functional analysis of recombinant channels in host cells using a fast agonist application system | |
dc.type | Chapter | |
dc.relation.ispartofseries-volume | 1677 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 163 | |
dc.source.id | SCOPUS10643745-2017-1677-SID85030786311 |