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dc.contributor.author | Coelho A. | |
dc.contributor.author | Martins T. | |
dc.contributor.author | Couto R. | |
dc.contributor.author | Deus C. | |
dc.contributor.author | Pereira C. | |
dc.contributor.author | Simões R. | |
dc.contributor.author | Rizvanov A. | |
dc.contributor.author | Silva F. | |
dc.contributor.author | Cunha-Oliveira T. | |
dc.contributor.author | Oliveira P. | |
dc.contributor.author | Serafim T. | |
dc.date.accessioned | 2018-04-05T07:09:25Z | |
dc.date.available | 2018-04-05T07:09:25Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0925-4439 | |
dc.identifier.uri | http://dspace.kpfu.ru/xmlui/handle/net/129730 | |
dc.description.abstract | © 2017 Elsevier B.V. Doxorubicin (DOX) is one of the most widely used anti-neoplastic agents. However, treatment with DOX is associated with cumulative cardiotoxicity inducing progressive cardiomyocyte death. Sirtuin 3 (Sirt3), a mitochondrial deacetylase, regulates the activity of proteins involved in apoptosis, autophagy and metabolism. Our hypothesis is that pharmacological modulation by berberine (BER) pre-conditioning of Sirt3 protein levels decreases DOX-induced cardiotoxicity. Our results showed that DOX induces cell death in all experimental groups. Increase in Sirt3 content by transfection-mediated overexpression decreased DOX cytotoxicity, mostly by maintaining mitochondrial network integrity and reducing oxidative stress. p53 was upregulated by DOX, and appeared to be a direct target of Sirt3, suggesting that Sirt3-mediated protection against cell death could be related to this protein. BER pre-treatment increased Sirt3 and Sirt1 protein levels in the presence of DOX and inhibited DOX-induced caspase 9 and 3-like activation. Moreover, BER modulated autophagy in DOX-treated H9c2 cardiomyoblasts. Interestingly, mitochondrial biogenesis markers were upregulated in in BER/DOX-treated cells. Sirt3 over-expression contributes to decrease DOX cytotoxicity on H9c2 cardiomyoblasts, while BER can be used as a modulator of Sirtuin function and cell quality control pathways to decrease DOX toxicity. | |
dc.relation.ispartofseries | Biochimica et Biophysica Acta - Molecular Basis of Disease | |
dc.subject | Berberine | |
dc.subject | Cardiotoxicity | |
dc.subject | Doxorubicin | |
dc.subject | Sirtuin 3 | |
dc.title | Berberine-induced cardioprotection and Sirt3 modulation in doxorubicin-treated H9c2 cardiomyoblasts | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 11 | |
dc.relation.ispartofseries-volume | 1863 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 2904 | |
dc.source.id | SCOPUS09254439-2017-1863-11-SID85030726008 |