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Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein—Barr Virus Latent Phase Protein LMP1

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dc.contributor.author Lomakin Y.
dc.contributor.author Shmidt A.
dc.contributor.author Bobik T.
dc.contributor.author Chernov A.
dc.contributor.author Pyrkov A.
dc.contributor.author Aleksandrova N.
dc.contributor.author Okunola D.
dc.contributor.author Vaskina M.
dc.contributor.author Ponomarenko N.
dc.contributor.author Telegin G.
dc.contributor.author Dubina M.
dc.contributor.author Belogurov A.
dc.date.accessioned 2018-04-05T07:08:52Z
dc.date.available 2018-04-05T07:08:52Z
dc.date.issued 2017
dc.identifier.issn 0007-4888
dc.identifier.uri http://dspace.kpfu.ru/xmlui/handle/net/129381
dc.description.abstract © 2017, Springer Science+Business Media, LLC. Intracellular fragments of latent phase protein LMP1 of Epstein—Barr virus, denoted as CTAR1/2/3, can trigger a variety of cell cascades and contribute to the transforming potential of the virus. Generation of recombinant proteins CTAR1/2/3 is expected to yield more ample data on functional and immunogenic characteristics of LMP1. We created genetic constructs for prokaryotic expression of LMP1 CTAR fragments and selected optimal conditions for their production and purification. Using a new library of LMP1 CTAR fragments, we carried out epitope mapping of a diagnostic anti-LMP1 antibody S12. Analysis of polyclonal serum antibodies from mice immunized with full-length LMP1 confirmed immunogenicity of CTAR elements comparable with that of full-length protein.
dc.relation.ispartofseries Bulletin of Experimental Biology and Medicine
dc.subject epitope detection
dc.subject latent membrane protein 1 (LMP1)
dc.subject prokaryotic expression
dc.subject S12 monoclonal antibody
dc.title Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein—Barr Virus Latent Phase Protein LMP1
dc.type Article
dc.relation.ispartofseries-issue 6
dc.relation.ispartofseries-volume 163
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 766
dc.source.id SCOPUS00074888-2017-163-6-SID85031932383


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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